Comparative stuy for LPS Extraction of NAG vibrio cholerae by EDTA, heating method and hot phenol method

Vibrio cholerae [NAG] was isolated from patients with diarrhea, identification by microbiological, biochemical and serological techniques, then Lip polysaccharide was extract from Vibrio cholerae [NAG][31G, 11G, 24G, 13G] by phenol and EDTA-heating method for comparison, The extracts were purified by Sepharose-4B gel. The results showed that EDTA-heating method was efficient and less expensive of time and material.

Numbers of plaque forming cells-IgG in peripheral blood of patients with chronic hepatitis B virus [HBV] infection

Numbers of PFC-IgG were enumerated in the peripheral blood of different groups of patients having chronic infection with HBV. In this study we included eight patients of chronic hepatitis B [CHB], seven HBV-healthy carriers, seven patients with autoimmune chronic active hepatitis [AICAH] that had many markers of HBV in their sera and ten healthy humans [control group] were investigated. A significantly [P<0.001] increased number of PFC-IgG in the peripheral blood of AICAH was observed as compared to the control group. No such difference was observed in the other two groups of patients [CHB and HBV-HC].